Microscopy and Microanalysis

Microscopy and Microanalysis
 
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JEOL 6400 SEM
JEOL 2011 STEM
JEOL 733 Microprobe
Leica Confocal LSM
Leica Digital LM
Skyscan 1072 Xray-CT
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LEICA TCS-SP8 CONFOCAL LSM

INSTRUMENTATION

Confocal Laser Scanning Microscope with:

  • Leica DMi8 Advanced inverted light microscope with motorized stage
  • LED bright field and EL6000 fluorescence light sources
  • 4 laser excitation wavelengths (nm): 405, 488, 552, and 638
  • 2 PMT SP fluorescence detectors, one HyD SP detector, transmitted light detector
  • LAS-X Software

LEICA TCS-SP2 CONFOCAL LSM


Zebrafish image: Dr. Bryan Crawford, Biology, UNB

INSTRUMENTATION

Confocal Laser Scanning Microscope with:

  • Leica DM IRE2 inverted stand IRE2 light microscope.
  • Wide selection of objective lenses including multi-immersion and long working-distance.
  • 4 lasers; Argon/Krypton; HeNe (green); HeNe (orange); HeNe (red).
  • 8 excitation wavelengths: 457, 476, 488, 496, 514, 543, 594, 633.
  • 5 detectors - 4 fluorescent channels plus one transmitted light detector.
  • AOBS (programmable beam splitter).
  • Physiology and materials software.

Rotating projections of composite confocal stacks through transgenic zebrafish embryos.
Green fluorescent protein (GFP) in developing endothelial cells.
Top embryo, 24 hpf; middle embryo 48 hpf; bottom embryo, 96 hpf.
Class of Biology 3181, UNB Biology, Winter 2011.


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